Description of Nupela indonesica sp. nov. (Bacillariophyceae) from freshwater ecosystems in Indonesia
1Maltsev Y.I., 2Kulikovskiy M.S.
1Bohdan Khmelnytskyi Melitopol State Pedagogical University, Melitopol, Ukraine
2Institute of Plant Physiology RAS, Moscow, Russia
e-mail: mz_5@ukr.net

Nupela indonesica sp. nov. (Bacillariophyceae) – новий вид діатомових водоростей із прісноводних екосистем Індонезії
1Мальцев Є.І., 2Куліковський М.С.
1Мелітопольський державний педагогічний університет ім. Б. Хмельницького, Мелітополь, Україна
2Інститут фізіології рослин ім. К.А. Тімірязєва, Москва, Росія
e-mail: mz_5@ukr.net

Annotation. For the first time, the phylogenetic position of the genus Nupela Vyverman & P.Compere is studied using molecular methods. In addition, we describe a new species, Nupela indonesica Kulikovskiy, Maltsev & Podunay sp. nov. from freshwater ecosystems in Indonesia, based on molecular and morphological investigations. The phylogenetic two-gene analysis of the genus Nupela shows that the new taxon is closely related to the genera Brachysira Kützing and Diadesmis Kützing.

The genus Nupela is a freshwater diatom genus established by Vyverman & Compère (1991) with N. giluwensis Vyverman & Compère, as the type species, described originally from high elevation ponds in Papua New Guinea. The genus Nupela does not show substrate preferences and it has been found on rocks, wood and saturated soils; being present both in slightly acidic and alkaline waters. At present, there are less than 100 species reported in this genus. However, phylogenetic position of this genus is uncertain. In this research, the morphology and ultrastructure of two new Nupela strains are presented. Studied planktonic samples were collected in Indonesia from a freshwater Lake Buyan (Bali Island) and a freshwater pond in Toraja (Sulawesi Island). The studies performed by light and scanning electron microscopy showed that the new isolates are an unknown taxon and so it is herein described as a new species, Nupela indonesica Kulikovskiy, Maltsev & Podunay sp. nov. The phylogenetic analysis (region V4 of 18S rDNA and partial rbcL gene) of the genus Nupela shows that the new taxon forms a separate clade, and with the low supports includes in a larger naviculoid clade with such genera as Diadesmis Kützing, Luticola D.G. Mann, Neidium Pfitzer, Scoliopleura Grunow and Brachysira Kützing.
The publication is based on research carried out with financial support provided by the RSF (14-14-00555).